Algal Culturing Techniques - download pdf or read online

By Robert A. Andersen

ISBN-10: 0120884267

ISBN-13: 9780120884261

Algal Culturing Techniques is a complete reference on all facets of the isolation and cultivation of marine and freshwater algae, together with seaweeds. it's divided into seven components that conceal historical past, media coaching, isolation and purification strategies, mass culturing recommendations, telephone counting and progress size innovations, and stories on themes and purposes of algal tradition concepts for environmental investigations.

Algal Culturing Techniques was once constructed to function either a brand new textbook and key reference for phycologists and others learning aquatic structures, aquaculture and environmental sciences. scholars of algal ecology, marine botany, marine phycology, and microbial ecology will benefit from the hands-on method for culturing numerous algae from clean and marine waters. Researchers in undefined, corresponding to aquaculture, pharmaceutical, foodstuffs, and biotechnology businesses will locate an authoritative and finished reference.

  • Sponsored via the Phycological Society of America
  • Features colour pictures and illustrations throughout
  • Describes culturing tools starting from the attempt tube to outdoors ponds and coastal seaweed farms
  • Details isolation recommendations starting from conventional micropipette to computerized circulation cytometeric methods
  • Includes purification, development, upkeep, and cryopreservation techniques
  • Highlights equipment for estimating algal populations, development premiums, keeping apart and measuring algal pigments, and detecting and culturing algal viruses
  • Features a finished appendix of approximately 50 algal tradition medium recipes
  • Includes a word list of phycological terms

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The choice of filter pore size is determined by the source of the seawater, its intended use, and the volume needed. 2 mm. 7 mm is recommended. Occasionally, dissolved organic matter removal may be required for special projects or because of suspected contamination. Dissolved organics may be removed by adsorption onto activated charcoal. The charcoal is prepared by washing with benzene, methanol, or 50% ethanol and distilled water (Craigie and McLachlan 1964). Dissolved organics in small volumes of seawater may be removed by adding 2 g of powdered, washed charcoal per liter of seawater, stirring for 1 hour and then filtering.

And Hepperle, D. 2000. Variability of calcification of Phacotus lenticularis (Chlorophyta, Chlamydomonadales) in nature and culture. Phycologia 39:318–22. Schlösser, U. G. 1994. SAG—Sammlung von Algenkulturen at the University of Göttingen catalogue of strains 1994. Bot. Acta 107:111–86. , and Nakano, M. 1989. Development of a solid medium for growth and isolation of axenic Microcystis strains (cyanobacteria). Appl. Environ. Microbiol. 55:2569–71. Skinner, C. E. 1932. Isolation in pure culture of green algae from soil by a simple technique.

1. 0. 1. 2. 3. 0. 1. 2. 3. 4. 5. 6. 7. 0. 1. 2. 0. 1. 2. 0. 0. References Natural seawater (NW) is a complex medium containing more than 50 known elements and a large and variable number of organic compounds. For algal culture, direct use of NW is seldom acceptable. Without the addition of further nutrients and trace metals, the yield of algae is usually too low for culture maintenance or laboratory experiments, and thus enrichment is normally required. 2). The preparation of NW with an enrichment solution of nutrients, trace metals, and vitamins and the preparation of AW are described in this chapter.

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Algal Culturing Techniques by Robert A. Andersen

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